umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Rho-kinase inhibitor Y-27632 and hypoxia synergistically enhance chondrocytic phenotype and modify S100 protein profiles in human chondrosarcoma cells
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-1732-4269
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Forskargrupp Daniel Marcellino)
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Forskargrupp Daniel Marcellino)ORCID iD: 0000-0002-4618-7267
Show others and affiliations
2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 3708Article in journal (Refereed) Published
Abstract [en]

Articular chondrocytes are slowly dividing cells that tend to lose their cell type-specific phenotype and ability to produce structurally and functionally correct cartilage tissue when cultured. Thus, culture conditions, which enhance the maintenance of chondrocyte phenotype would be very useful for cartilage research. Here we show that Rho-kinase inhibition by Y-27632 under hypoxic conditions efficiently maintains and even enhances chondrocyte-specific extracellular matrix production by chondrocytic cells. The effects of long-term Y-27632 exposure to human chondrosarcoma 2/8 cell phenotype maintenance and extracellular matrix production were studied at normoxia and at a 5% low oxygen atmosphere. Y-27632 treatment at normoxia induced ACAN and COL2A1 gene up-regulation and a minor increase of sulfated glycosaminoglycans (sGAGs), while type II collagen expression was not significantly up-regulated. A further increase in expression of ACAN and COL2A1 was achieved with Y-27632 treatment and hypoxia. The production of sGAGs increased by 65.8%, and ELISA analysis revealed a 6-fold up-regulation of type II collagen. Y-27632 also induced the up-regulation of S100-A1 and S100-B proteins and modified the expression of several other S100 protein family members, such as S100-A4, S100-A6, S100-A13 and S100-A16. The up-regulation of S100-A1 and S100-B proteins is suggested to enhance the chondrocytic phenotype of these cells.

Place, publisher, year, edition, pages
London: Nature Publishing Group, 2017. Vol. 7, 3708
Keyword [en]
Chondrocyte, S-100 proteins, Rho-kinase inhibition, extracellular matrix
National Category
Cell and Molecular Biology Orthopedics Cell Biology
Research subject
Biochemistry; cellforskning; Molecular Medicine
Identifiers
URN: urn:nbn:se:umu:diva-136633DOI: 10.1038/s41598-017-03958-5ISI: 000403413700070PubMedID: 28623370OAI: oai:DiVA.org:umu-136633DiVA: diva2:1112732
Available from: 2017-06-20 Created: 2017-06-20 Last updated: 2017-07-18Bibliographically approved

Open Access in DiVA

fulltext(9561 kB)1 downloads
File information
File name FULLTEXT01.pdfFile size 9561 kBChecksum SHA-512
2843980b912d1771c7f0a8401146619a1be790f2fa5cd7d305b55ab94b1b2f0ab2dd321c710a05b3491c2fa9b72dfcdf122cb1ee777e65fb66bc505750a96955
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Piltti, JuhaBygdell, JoakimFernández-Echevarría, CeciliaMarcellino, DanielLammi, Mikko
By organisation
Department of Integrative Medical Biology (IMB)Department of Chemistry
In the same journal
Scientific Reports
Cell and Molecular BiologyOrthopedicsCell Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf