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Recombinant expression of Mimivirus L725 ORFan gene product
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2017 (English)In: Acta virologica, ISSN 0001-723X, E-ISSN 1336-2305, Vol. 61, no 1, 123-126 p.Article in journal (Refereed) Published
Abstract [en]

Mimivirus was the first discovered amoebal giant virus. The Mimivirus virions are covered by a dense layer of approximately 130 nm-long fibers, the length and shape of which diverge from those of other viruses. Here, we aimed at expressing the L725 protein to further confirm and study its role as a fiber-associated protein. We report Escherichia coli expression of the L725 protein, which is encoded by a Mimivirus ORFan, was previously identified by proteomics in purified viral fibers and demonstrated to be a fiber-associated protein by RNA-silencing experiments. The expressed protein was recognized by anti-Mimivirus fiber or anti-Mimivirus L725 polyclonal antibodies. This study is the only expression, to our knowledge, of a product from a Mimiviral ORFan gene.

Place, publisher, year, edition, pages
AEPRESS SRO , 2017. Vol. 61, no 1, 123-126 p.
Keyword [en]
Mimivirus, giant virus, fiber, ORFan, gene expression
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-137025DOI: 10.4149/av_2017_01_123ISI: 000401911800014PubMedID: 28161966OAI: oai:DiVA.org:umu-137025DiVA: diva2:1117497
Available from: 2017-06-29 Created: 2017-06-29 Last updated: 2017-06-29Bibliographically approved

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