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Gene expression profiles and molecular mechanism of cultured human chondrocytes' exposure to T-2 toxin and deoxynivalenol
School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an, PR China.
School of Clinical Medicine, Hainan Medical University, Haikou, PR China.
Hong Hui Hospital, Health Science Center, Xi'an Jiaotong University, Xi'an, PR China.
School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an, PR China.
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2017 (English)In: Toxicon, ISSN 0041-0101, E-ISSN 1879-3150, Vol. 140, 38-44 p.Article in journal (Refereed) Published
Abstract [en]

T-2 toxin and deoxynivalenol (DON) are secondary metabolites produced by Fusarium fungi and are commonly found on food and feed. Although T-2 toxin and DON have been suggested as the etiology of Kashin-Beck disease (KBD), an endemic osteochondropathy, little is known about the mechanism when human chondrocytes are exposed to T-2 toxin and DON. The purpose of this study is to identify the gene expression differences and underlying molecular changes modulated by T-2 toxin and DON in vitro in human chondrocytes. After the experiments of cell viability, the gene expression profiles were analyzed in cells that were treated with 0.01 μg/ml T-2 toxin and 1.0 μg/ml DON for 72 h by Affymetrix Human Gene Chip. The array results showed that 882 and 2118 genes were differentially expressed for T-2 toxin and DON exposure, respectively. Enrichment analysis revealed that diverse cellular processes including DNA damage, cell cycle regulation and metabolism of extracellular matrix were affected when human chondrocytes were exposed to T-2 toxin and DON. These results demonstrate the gene expression differences and molecular mechanism of cultured human chondrocytes exposure to T-2 toxin and DON, and provide a new insight into future research in the etiology of KBD.

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2017. Vol. 140, 38-44 p.
Keyword [en]
Deoxynivalenol, Human chondrocytes, Microarray, T-2 toxin
National Category
Cell and Molecular Biology Environmental Health and Occupational Health Pharmacology and Toxicology
Research subject
cellforskning; Pathology; Molecular Biology; Toxicology
Identifiers
URN: urn:nbn:se:umu:diva-137761DOI: 10.1016/j.toxicon.2017.06.014PubMedID: 28684119OAI: oai:DiVA.org:umu-137761DiVA: diva2:1121645
Available from: 2017-07-12 Created: 2017-07-12 Last updated: 2017-12-04Bibliographically approved

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