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Effects of long-term hypoxia in human chondrosarcoma cells
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-1732-4269
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0002-9833-4628
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-1710-7715
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). School of Public Health, Health Science Center, Xi’an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi’an, China. (Chondrogenic and Osteogenic Differentiation Group)ORCID iD: 0000-0002-6181-9904
2018 (English)In: Journal of Cellular Biochemistry, ISSN 0730-2312, E-ISSN 1097-4644, Vol. 119, no 2, p. 2320-2332Article in journal (Refereed) Published
Abstract [en]

The cell-based therapies could be potential methods to treat damaged cartilage tissues. Instead of native hyaline cartilage, the current therapies generate mainly weaker fibrocartilage-type of repair tissue. A correct microenvironment influences the cellular phenotype, and together with external factors it can be used, e.g., to aid the differentiation of mesenchymal stem cells to defined types of differentiated adult cells. In this study, we investigated the effect of long-term exposure to 5% low oxygen atmosphere on human chondrosarcoma HCS-2/8 cells. This atmosphere is close to normal oxygen tension of cartilage tissue. The proteome was analyzed with label-free mass spectrometric method and further bioinformatic analysis. The qRT-PCR method was used to gene expression analysis, and ELISA and dimethylmethylene blue assays for type II collagen and sulfated glycosaminoglycan measurements. The hypoxic atmosphere did not influence cell proliferation, but enhanced slightly ACAN and COL2A1 gene expression. Proteomic screening revealed a number of hypoxia-induced protein level responses. Increased ones included NDUFA4L2, P4HA1, NDRG1, MIF, LDHA, PYGL, while TXNRD1, BAG2, TXN2, AQSTM1, TNFRSF1B and EPHX1 decreased during the long-term hypoxia. Also a number of proteins previously not related to hypoxia changed during the treatment. Of those S100P, RPSS26, NDUFB11, CDV3 and TUBB8 had elevated levels, while ALCAM, HLA-B, EIF1, and ACOT9 were lower in the hypoxia samples. In conclusion, low oxygen condition causes changes in the cellular amounts of several proteins.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018. Vol. 119, no 2, p. 2320-2332
Keywords [en]
chondrosarcoma, extracellular matrix, hypoxia, label-free quantitative proteomics, S100 proteins
National Category
Cell Biology Cell and Molecular Biology
Research subject
Biochemistry; cellforskning; Medical Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-138929DOI: 10.1002/jcb.26394ISI: 000418708300098PubMedID: 28865129OAI: oai:DiVA.org:umu-138929DiVA, id: diva2:1138177
Funder
Swedish Rheumatism Association, R-567071Available from: 2017-09-04 Created: 2017-09-04 Last updated: 2018-06-09Bibliographically approved

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Piltti, JuhaBygdell, JoakimQu, ChenjuanLammi, Mikko

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