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Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2017 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 987, p. 15-24Article in journal (Refereed) Published
Abstract [en]

This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The EIS measurements of MTX resulted in a data matrix of size 39 × 55. The data were analysed using multivariate data analysis and showed a concentration dependence and time dependence that could be separated. This allowed the calculation of a multivariate calibration model. The model showed good linear behavior on a logarithmic scale offering a detection limit of 5 × 10−12 mol L−1.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 987, p. 15-24
Keywords [en]
Gold electrode, Biosensor, X-ray photoelectron spectroscopy, Singular value decomposition, Multivariate calibration model, Antibody
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-138962DOI: 10.1016/j.aca.2017.08.034ISI: 000410304700002PubMedID: 28916036OAI: oai:DiVA.org:umu-138962DiVA, id: diva2:1138350
Available from: 2017-09-05 Created: 2017-09-05 Last updated: 2018-06-09Bibliographically approved

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Phal, SereilakhenaLindholm-Sethson, BrittaShchukarev, AndreyTesfalidet, Solomon

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