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Active uptake of kleptoplastids by Dinophysis caudata from its ciliate prey Myrionecta rubra
Linnéuniversitetet, Institutionen för naturvetenskap, NV. (EcoChange)
Chonnam National University, Republic of Korea.
Linnéuniversitetet, Institutionen för naturvetenskap, NV.
Kunsan National University, Republic of Korea.
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2011 (English)In: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 62, no 1, p. 99-108Article in journal (Refereed) Published
Abstract [en]

We verified an active uptake of kleptoplastids in the toxic and bloom-forming dinoflagellatesof the genus Dinophysis from its preferred prey, the ciliate Myrionecta rubra, using a quantitativereal-time PCR technique. During a 65 d starvation/feeding experiment with Dinophysis caudata,changes in plastid 16S rRNA, plastid autofluorescence and plastid/nuclear DNA ratio throughthe cell cycle were followed with quantitative real-time PCR and flow cytometry. During starvation,the cultures initially showed a rapid growth and a 3.5-fold increase of number of cells ml–1, while atthe same time, plastid DNA cell–1 showed a 3.5-fold decrease, and a 3.6-fold decrease in phycoerythrinfluorescence cell–1. The decrease in plastid DNA cell–1 d–1 closely followed culture growth rate(Pearson correlation, r = 0.91), indicating that existing plastids were diluted within the growing populationand that no new plastids were synthesised by the cells. When starved cells were re-fed by theciliate M. rubra on Days 43 to 51 of the experiment, plastid DNA cell–1 increased 7-fold up to 14 00016S DNA copies per cell, thereby directly revealing the kleptoplastic behaviour. The implication isthat not only availability of the prey M. rubra itself, but also the supply of suitable kleptoplastidsmight be an important controlling factor for Dinophysis spp. bloom formation and decline.

Place, publisher, year, edition, pages
2011. Vol. 62, no 1, p. 99-108
National Category
Ecology
Research subject
Ecology, Aquatic Ecology
Identifiers
URN: urn:nbn:se:umu:diva-139419DOI: 10.3354/ame01459OAI: oai:DiVA.org:umu-139419DiVA, id: diva2:1140404
Available from: 2011-02-07 Created: 2017-09-12

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