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Digital holographic imaging with plane wavefronts - assessing the diffusing constant of a microparticle
Umeå University, Faculty of Science and Technology, Department of Physics.
2017 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

We built a digital holographic microscopy (DHM) system with a camera, an objective, lenses, and reconstruction algorithm. Then, we investigated the accuracy by calculating the error of diffusion coefficient of the one-micrometer microspheres from their holograms. By obtaining the position of the particle in x, y axis from the center of the particle images in the holograms and reconstructing the holograms of the particle for its position in the z axis, we got the 3D trajectory of the microspheres to compute the diffusion coefficient. Our calculations show that the diffusion coefficient of the particle to be 5.228 x 10-13, 4.605 x 10-13 and 7.506 x 10-13 m2s-1 for x, y and z axis respectively. While the result of z axis is bigger than the theoretical value, x and y axis have smaller error. The results reveal that DHM method is more easily prone to the systematic error than traditional microscopy.

Place, publisher, year, edition, pages
2017.
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-142022OAI: oai:DiVA.org:umu-142022DiVA, id: diva2:1157881
Subject / course
Fysik C - Examensarbete
Available from: 2017-11-17 Created: 2017-11-16 Last updated: 2017-11-17Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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