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Development of a digital holographic imaging system using a point source - investigation to microparticle diffusion
Umeå University, Faculty of Science and Technology, Department of Physics.
2017 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Digital holographic microscopy (DHM) is a tool that can be used to acquire the 3D information of the micro-sized particles using their 2D holograms. The aim of this paper was to investigate the diffusion of micro-particles based on DHM technology. We built up a DHM system consists of a point source using laser and pinhole, a movable sample stage in three dimensions, and an imaging system that capture holograms of micro-particles. We also implemented an algorithm for hologram reconstruction in MATLAB. In the experiment, we verified our algorithm by using synthetic images and the results showed that the algorithm was correct. When applied our algorithm to study the diffusion of particles, we found that the diffusion constant could be calculated easily based on the 3D positions of particles. The calculated constants are 3.02x10-13m2/s, 2.96x10-13m2/s and 3.44x10-13 m2/s in x, y, z axis, respectively. And the theoretical value is 4.64x10-13m2/s. In conclusion, it is feasible to use our DHM system to investigate the diffusion of the micro-particles.

Place, publisher, year, edition, pages
2017.
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-142024OAI: oai:DiVA.org:umu-142024DiVA, id: diva2:1157884
Subject / course
Fysik C - Examensarbete
Available from: 2017-11-17 Created: 2017-11-16 Last updated: 2017-11-17Bibliographically approved

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CiteExportLink to record
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