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Inhibition of curli assembly and Escherichia coli biofilm formation by the human systemic amyloid precursor transthyretin
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2017 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 114, no 46, p. 12184-12189Article in journal (Refereed) Published
Abstract [en]

During biofilm formation, Escherichia coli and other Enterobacteriaceae produce an extracellular matrix consisting of curli amyloid fibers and cellulose. The precursor of curli fibers is the amyloidogenic protein CsgA. The human systemic amyloid precursor protein transthyretin (TTR) is known to inhibit amyloid-β (Aβ) aggregation in vitro and suppress the Alzheimer’s-like phenotypes in a transgenic mouse model of Aβ deposition. We hypothesized that TTR might have broad antiamyloid activity because the biophysical properties of amyloids are largely conserved across species and kingdoms. Here, we report that both human WT tetrameric TTR (WT-TTR) and its engineered nontetramer-forming monomer (M-TTR, F87M/L110M) inhibit CsgA amyloid formation in vitro, with M-TTR being the more efficient inhibitor. Preincubation of WT-TTR with small molecules that occupy the T4 binding site eliminated the inhibitory capacity of the tetramer; however, they did not significantly compromise the ability of M-TTR to inhibit CsgA amyloidogenesis. TTR also inhibited amyloid-dependent biofilm formation in two different bacterial species with no apparent bactericidal or bacteriostatic effects. These discoveries suggest that TTR is an effective antibiofilm agent that could potentiate antibiotic efficacy in infections associated with significant biofilm formation.

Place, publisher, year, edition, pages
2017. Vol. 114, no 46, p. 12184-12189
Keyword [en]
Amyloids, CsgA, Transthyretin, Biofilms, Curli
National Category
Chemical Sciences Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-142602DOI: 10.1073/pnas.1708805114ISI: 000415173300053PubMedID: 29087319OAI: oai:DiVA.org:umu-142602DiVA, id: diva2:1162975
Available from: 2017-12-05 Created: 2017-12-05 Last updated: 2018-06-09Bibliographically approved

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Ådén, JörgenAlmqvist, Fredrik

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