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Evaluation of six commercially available kits using purified heterophile antigen for the rapid diagnosis of infectious mononucleosis compared with Epstein-Barr virus-specific serology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology. (Fredrik Elgh)
1996 (English)In: Clinical and Diagnostic Virology, ISSN 0928-0197, E-ISSN 1873-4901, Vol. 7, no 1, p. 17-21Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Novel commercial kits based on antibody reactivity to purified heterophile antigens have recently been introduced for the diagnosis of Epstein-Barr (EB) virus-associated infectious mononucleosis (IM). It is important to determine possible improvements in the performance and reliability of such tests for the diagnosis of IM.

OBJECTIVE: To evaluate the reliability of six commercially available kits for the rapid diagnosis of IM in comparison to EB-virus-specific serology.

STUDY DESIGN: In total, 100 sera, 53 from patients with serologically verified primary EB virus infection and 47 from EB-virus-immune or -susceptible patients, were used to evaluate the six rapid test kits: Monolatex, Mono-Latex, Mono-Lex (latex agglutination-based kits), Mono-Plus, IM-Check and Clearview IM (solid-phase-based kits). EB-virus-specific serologies including detection of viral capsid antigen IgM and IgG and EB nuclear antigen-1 IgG, were used as reference methods.

RESULTS: Compared with the reference methods, the sensitivities and specificities of the heterophile antibody test kits were 70-92% and 96-100%, respectively. IM-Check had a low sensitivity and was difficult to read. The remaining kits performed well.

CONCLUSION: Monolatex, Mono-Latex, Mono-Lex, Mono-Plus and Clearview IM can be recommended for the confirmation of EB-virus-associated infectious mononucleosis. Clearview IM combined a high sensitivity and specificity with very simple one-step solid-phase-based procedure.

Place, publisher, year, edition, pages
1996. Vol. 7, no 1, p. 17-21
Identifiers
URN: urn:nbn:se:umu:diva-143293PubMedID: 9077426OAI: oai:DiVA.org:umu-143293DiVA, id: diva2:1168249
Available from: 2017-12-20 Created: 2017-12-20 Last updated: 2018-06-09

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CiteExportLink to record
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