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Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica).
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology. (Fredrik Elgh)
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1997 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 35, no 12, p. 3264-8Article in journal (Refereed) Published
Abstract [en]

Nephropathia epidemica (NE), Puumala (PUU) virus infection, is a febrile disease which is commonly associated with acute renal impairment. To differentiate NE from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. In the present report we describe a comparative evaluation of six PUU virus immunoglobulin M (IgM) and seven IgG enzyme-linked immunosorbent assay (ELISA) protocols based on native, Escherichia coli-expressed, or baculovirus-expressed nucleocapsid protein (N). Neutralization and immunofluorescence assays were included for comparison. Equally high sensitivities and specificities were obtained with three mu-capture-based IgM ELISAs using native, baculovirus-expressed, and E. coli-expressed N antigens, respectively, and by an ELISA based on purified E. coli-expressed full-length N adsorbed to solid phase. The assays based on truncated amino-terminal N proteins, including a commercially available PUU virus IgM ELISA, all showed lower sensitivities. For detection of PUU virus-specific IgG, ELISAs based on monoclonal antibody-captured native or baculovirus-expressed N antigens showed optimal sensitivities and specificities, while the assays based on E. coli-expressed N did not detect all PUU virus IgG-positive serum samples. A commercially available PUU virus IgG ELISA based on E. coli-expressed amino-terminal N showed a significantly lower sensitivity than those of all other IgG assays.

Place, publisher, year, edition, pages
1997. Vol. 35, no 12, p. 3264-8
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URN: urn:nbn:se:umu:diva-143301PubMedID: 9399531OAI: oai:DiVA.org:umu-143301DiVA, id: diva2:1168262
Available from: 2017-12-20 Created: 2017-12-20 Last updated: 2018-06-09

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CiteExportLink to record
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  • apa
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