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Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
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2018 (English)In: Journal of Electroanalytical Chemistry, ISSN 1572-6657Article in journal (Refereed) Published
Abstract [en]

Abstract The classical way to modify a gold electrode surface for immunosensor development is through self-assembly of functionalised thiols for subsequent attachment of antibodies. Here is described a new pathway for surface modification using a diazonium salt-based immunosensor for detection of methotrexate (MTX) with electrochemical immittance spectroscopy (EIS). The diazonium salt, 4-carboxybenzenediazonium tetrafluoroborate was synthesized using a diazotization reaction and characterized by IR and cyclic voltammetry (CV). The Au electrode was electrografted with diazonium and used for anti-MTX antibody immobilization. The EIS detection of MTX was studied with and without application of redox probe; Fe(CN)63−/4−. MTX could not be detected using Fe(CN)63−/4−as redox probe, whereas EIS measurements without redox probe and analysis of the data with singular decomposition (SVD) gave good results. A multivariate calibration model showed good linear behavior on a logarithmic scale with a detection limit for MTX of 7×10−12molL−1.

Place, publisher, year, edition, pages
Elsevier, 2018.
Keyword [en]
4-carboxybenzenediazonium tetrafluoroborate, Gold electrode, Antibody, Immunosensor, Singular value decomposition, Cyclic voltammetry, Chronoamperometry, Electrochemical impedance spectroscopy
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-143882DOI: 10.1016/j.jelechem.2017.12.072OAI: oai:DiVA.org:umu-143882DiVA, id: diva2:1173462
Available from: 2018-01-12 Created: 2018-01-12 Last updated: 2018-06-09

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S1572665717309530

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Phal, SereilakhenaShatri, BesartLindholm-Sethson, BrittaTesfalidet, Solomon

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Phal, SereilakhenaShatri, BesartLindholm-Sethson, BrittaTesfalidet, Solomon
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CiteExportLink to record
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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
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  • Other style
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  • de-DE
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  • nn-NO
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