umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Photosynthetic growth despite a broken Q-cycle
2011 (English)In: Nat Commun, Vol. 2, p. 301-306Article in journal (Refereed) Published
Abstract [en]

Central in respiration or photosynthesis, the cytochrome bc(1) and b(6)f complexes are regarded as functionally similar quinol oxidoreductases. They both catalyse a redox loop, the Q-cycle, which couples electron and proton transfer. This loop involves a bifurcated electron transfer step considered as being mechanistically mandatory, making the Q-cycle indispensable for growth. Attempts to falsify this paradigm in the case of cytochrome bc(1) have failed. The rapid proteolytic degradation of b(6)f complexes bearing mutations aimed at hindering the Q-cycle has precluded so far the experimental assessment of this model in the photosynthetic chain. Here we combine mutations in Chlamydomonas that inactivate the redox loop but preserve high accumulation levels of b(6)f complexes. The oxidoreductase activity of these crippled complexes is sufficient to sustain photosynthetic growth, which demonstrates that the Q-cycle is dispensable for oxygenic photosynthesis.

Place, publisher, year, edition, pages
2011. Vol. 2, p. 301-306
Keywords [en]
Benzoquinones, Chlamydomonas reinhardtii/*enzymology/genetics/growth & development, Cytochrome b6f Complex/analysis/genetics/*metabolism, Cytochromes f/metabolism, Electron Transport, Heme/deficiency, Hydroquinones, Immunoblotting, Mutation, Oxidoreductases/*metabolism, Photosynthesis/*physiology
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:umu:diva-144809ISBN: 2041-1723 (Electronic) 2041-1723 (Linking) OAI: oai:DiVA.org:umu-144809DiVA, id: diva2:1182878
Note

Malnoe, Alizee Wollman, Francis-Andre de Vitry, Catherine Rappaport, Fabrice eng Research Support, Non-U.S. Gov't England 2011/05/11 06:00 Nat Commun. 2011;2:301. doi: 10.1038/ncomms1299.

Available from: 2018-02-14 Created: 2018-02-14 Last updated: 2018-06-09

Open Access in DiVA

No full text in DiVA

Other links

http://www.ncbi.nlm.nih.gov/pubmed/21556055

Authority records BETA

Malnoë, Alizée

Search in DiVA

By author/editor
Malnoë, Alizée
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 11 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf