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Distinct roles of the photosystem II protein PsbS and zeaxanthin in the regulation of light harvesting in plants revealed by fluorescence lifetime snapshots
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2014 (English)In: Proc Natl Acad Sci USA, Vol. 111, no 49, p. 17498-503Article in journal (Refereed) Published
Abstract [en]

The photosystem II (PSII) protein PsbS and the enzyme violaxanthin deepoxidase (VDE) are known to influence the dynamics of energy-dependent quenching (qE), the component of nonphotochemical quenching (NPQ) that allows plants to respond to fast fluctuations in light intensity. Although the absence of PsbS and VDE has been shown to change the amount of quenching, there have not been any measurements that can detect whether the presence of these proteins alters the type of quenching that occurs. The chlorophyll fluorescence lifetime probes the excited-state chlorophyll relaxation dynamics and can be used to determine the amount of quenching as well as whether two different genotypes with the same amount of NPQ have similar dynamics of excited-state chlorophyll relaxation. We measured the fluorescence lifetimes on whole leaves of Arabidopsis thaliana throughout the induction and relaxation of NPQ for wild type and the qE mutants, npq4, which lacks PsbS; npq1, which lacks VDE and cannot convert violaxanthin to zeaxanthin; and npq1 npq4, which lacks both VDE and PsbS. These measurements show that although PsbS changes the amount of quenching and the rate at which quenching turns on, it does not affect the relaxation dynamics of excited chlorophyll during quenching. In addition, the data suggest that PsbS responds not only to DeltapH but also to the Deltapsi across the thylakoid membrane. In contrast, the presence of VDE, which is necessary for the accumulation of zeaxanthin, affects the excited-state chlorophyll relaxation dynamics.

Place, publisher, year, edition, pages
2014. Vol. 111, no 49, p. 17498-503
Keywords [en]
Arabidopsis/enzymology, Arabidopsis Proteins/*metabolism, Chlorophyll/chemistry, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Genes, Plant/genetics, *Light, Light-Harvesting Protein Complexes/*metabolism, Oxidoreductases/metabolism, Photosystem II Protein Complex/*metabolism, Zeaxanthins/*metabolism, PsbS, carotenoids, fluorescence lifetime, nonphotochemical quenching, photosystem II
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:umu:diva-144806ISBN: 1091-6490 (Electronic) 0027-8424 (Linking) OAI: oai:DiVA.org:umu-144806DiVA, id: diva2:1183507
Note

Sylak-Glassman, Emily J Malnoe, Alizee De Re, Eleonora Brooks, Matthew D Fischer, Alexandra Lee Niyogi, Krishna K Fleming, Graham R eng Howard Hughes Medical Institute/ Research Support, Non-U.S. Gov't 2014/11/26 06:00 Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):17498-503. doi: 10.1073/pnas.1418317111. Epub 2014 Nov 24.

Available from: 2018-02-17 Created: 2018-02-17 Last updated: 2018-06-09

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http://www.ncbi.nlm.nih.gov/pubmed/25422428

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Malnoë, A.

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