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Crystallization of recombinant α-actinin and related proteins
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.ORCID iD: 0000-0003-3044-1256
2018 (English)In: Schizosaccharomyces pombe: methods and protocols / [ed] Teresa L. Singleton, Humana Press, 2018, p. 95-103Chapter in book (Other academic)
Abstract [en]

When it comes to crystallization each protein is unique. It can never be predicted beforehand in which condition the particular protein will crystallize or even if it is possible to crystallize. Still, by following some simple checkpoints the chances of obtaining crystals are increased. The primary checkpoints are purity, stability, concentration, and homogeneity. High-quality protein crystals are needed. This protocol will allow an investigator to: clone, express, and crystallize a protein of interest.

Place, publisher, year, edition, pages
Humana Press, 2018. p. 95-103
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1721
Keywords [en]
Protein purification, Crystallization, Optimization
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-145559DOI: 10.1007/978-1-4939-7546-4_9ISBN: 978-1-4939-7545-7 (print)ISBN: 978-1-4939-7546-4 (print)OAI: oai:DiVA.org:umu-145559DiVA, id: diva2:1189410
Available from: 2018-03-09 Created: 2018-03-09 Last updated: 2019-03-14Bibliographically approved

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Persson, KarinaBackman, Lars

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