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Cooke-Triplet-Tweezers: More compact, robust and efficient optical tweezers
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Science and Technology, Department of Physics.ORCID iD: 0000-0002-3261-9903
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2018 (English)In: Optics Letters, ISSN 0146-9592, E-ISSN 1539-4794, Vol. 43, no 9, p. 1990-1993Article in journal (Refereed) Published
Abstract [en]

We present a versatile three-lens optical design to improve the overall compactness, efficiency, and robustness for optical tweezers based applications. The design, inspired by the Cooke–Triplet configuration, allows for continuous beam magnifications of 2–10× , and axial as well as lateral focal shifts can be realized without switching lenses or introducing optical aberrations. We quantify the beam quality and trapping stiffness and compare the Cooke–Triplet design with the commonly used double Kepler design through simulations and direct experiments. Optical trapping of 1 and 2 μm beads shows that the Cooke–Triplet possesses an equally strong optical trap stiffness compared to the double Kepler lens design but reduces its lens system length by a factor of 2.6. Finally, we demonstrate how a Twyman–Green interferometer integrated in the Cooke–Triplet optical tweezers setup provides a fast and simple method to characterize the wavefront aberrations in the lens system and how it can help in aligning the optical components perfectly.

Place, publisher, year, edition, pages
2018. Vol. 43, no 9, p. 1990-1993
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-145899DOI: 10.1364/OL.43.001990ISI: 000431179400013PubMedID: 29714728Scopus ID: 2-s2.0-85046663259OAI: oai:DiVA.org:umu-145899DiVA, id: diva2:1191927
Available from: 2018-03-21 Created: 2018-03-21 Last updated: 2023-09-07Bibliographically approved
In thesis
1. KNOW YOUR ENEMY: Characterizing Pathogenic Biomaterials Using Laser Tweezers
Open this publication in new window or tab >>KNOW YOUR ENEMY: Characterizing Pathogenic Biomaterials Using Laser Tweezers
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Diseases caused by pathogenic agents such as bacteria and viruses result in devastating costs on personal and societal levels. However, it is not just the emergence of new diseases that is problematic. Antibiotic resistance among bacteria makes uncomplicated infections difficult and lethal. Resilient disease-causing spores spread in hospitals, the food industry, and water supplies requiring effective detection and disinfection methods. Further, we face complex neurological diseases where no effective treatment or diagnostic methods exist. Thus, we must increase our fundamental understanding of these diseases to develop effective diagnostic, detection, disinfection, and treatment methods.

Classically, the methods used for detecting and studying the underlying mechanics of pathogenic agents work on a large scale, measuring the average macroscopic behavior and properties of these pathogens. However, just as with humans, the average behavior is not always representative of individual behavior. Therefore, it is also essential to investigate the characteristics of these pathogens on a single cell or particle level. 

This thesis develops and applies optical techniques to characterize pathogenic biomaterial on a single cell or particle level. At the heart of all these studies is our Optical Tweezers (OT) instrument. OT are a tool that allows us to reach into the microscopic world and interact with it. Finally, by combining OT with other experimental techniques, we can chemically characterize biomaterials and develop assays that mimic different biological settings. Using these tools, we investigate bacterial adhesion, disinfection, and detection of pathogenic spores and proteins.

Hopefully, the insights of these studies can lessen the burden on society caused by diseases by helping others develop effective treatment, diagnostic, detection, and disinfection methods in the future. 

Place, publisher, year, edition, pages
Umeå: Umeå universitet, 2022. p. 73
Keywords
Optical Tweezers, Laser Tweezers, Raman Spectroscopy, Bacterial Adhesion, Biophysics, Pili, Bacterial Spores, Endospores, Oocysts, Cryptosporidium, Optics
National Category
Biophysics Atom and Molecular Physics and Optics
Research subject
biology; Physics
Identifiers
urn:nbn:se:umu:diva-192471 (URN)978-91-7855-726-4 (ISBN)978-91-7855-727-1 (ISBN)
Public defence
2022-03-11, NAT.D.410, Naturvetarhuset, Umeå, 09:00 (English)
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Supervisors
Available from: 2022-02-18 Created: 2022-02-14 Last updated: 2022-02-15Bibliographically approved

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Stangner, TimDahlberg, TobiasSvenmarker, PontusZakrisson, JohanWiklund, KristerAndersson, Magnus

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