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Comparative exoproteome analyses of Lactobacillus spp. reveals species-and strain-specific proteins involved in their extracellular interaction and probiotic potential
Dankook University.
Dankook University.ORCID iD: 0000-0002-4396-346X
Dankook University.
Dankook University.
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2018 (English)In: Lebensmittel-Wissenschaft + Technologie, ISSN 0023-6438, E-ISSN 1096-1127, Vol. 93, p. 420-426Article in journal (Refereed) Published
Abstract [en]

Due to their health-promoting effects, the probiotic applications of lactobacilli have been investigated. Aside from their basic cellular functions, the extracellular activities of probiotic lactobacilli influence their establishment and interaction with the host. Many extracellular proteins play important roles in bacterial colonization and survival in their host's gastrointestinal environment. In this study, we compared the exoproteome of three lactobacillus strains isolated from the gastrointestinal tracts of pigs and chickens, namely, Lactobacillus mucosae LM1, Ljohnsonii PF01, and Ljohnsonii C1–10. Extracellular proteins collected in the mid-logarithmic growth phase were identified and quantified using a Q Exactive™ Orbitrap mass spectrometer (MS). Of 99 total extracellular proteins, 83% belonged to Lmucosae LM1; Ljohnsonii PF01 strains had fewer extracellular proteins. Enolase, which is involved in the glycolysis pathway and has moonlighting functions in the adhesion of probiotic bacteria, was found in the core exoproteomes of the three strains. The most abundant proteins of each strain were phosphoketolase in Lmucosae LM1, aggregation-promoting factor in Ljohnsonii PF01, and levansucrase in Ljohnsonii C1–10. The observed differences between these three exoproteomes in terms of cellular and molecular function elucidate the extracellular activities of these isolates.

Place, publisher, year, edition, pages
2018. Vol. 93, p. 420-426
Keywords [en]
Extracellular proteome, Lactobacillus johnsonii, Lactobacillus mucosae, Lactobacilli, Probiotics
National Category
Agricultural and Veterinary sciences
Identifiers
URN: urn:nbn:se:umu:diva-146378DOI: 10.1016/j.lwt.2018.03.069ISI: 000432640600058OAI: oai:DiVA.org:umu-146378DiVA, id: diva2:1195880
Available from: 2018-04-06 Created: 2018-04-06 Last updated: 2018-12-11Bibliographically approved

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Valeriano, Valerie Diane V.

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