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A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4
Umeå University, Faculty of Science and Technology, Department of Physics.
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2018 (English)In: Australian journal of chemistry (Print), ISSN 0004-9425, E-ISSN 1445-0038, Vol. 71, no 2-3, p. 170-176Article in journal (Refereed) Published
Abstract [en]

A novel aptasensor based on a tetracycline (TET) aptamer immobilized by physical adsorption on an ordered mesoporous carbon-Fe3O4 (OMC-Fe3O4)-modified screen-printed electrode surface was successfully fabricated. OMC-Fe3O4 was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The modification procedure of the aptasensor was characterized by cyclic voltammetry. Interaction between the TET aptamer and target was determined by differential pulse voltammetry. Under optimal conditions, the proposed aptasensor exhibited good electrochemical sensitivity to TET in a concentration range of 5 nM to 10 μM, with a detection limit of 0.8 nM (S/N =3D 3). This aptasensor exhibited satisfactory specificity, reproducibility, and stability.

Place, publisher, year, edition, pages
CSIRO Publishing, 2018. Vol. 71, no 2-3, p. 170-176
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-146171DOI: 10.1071/CH17503ISI: 000426369100012OAI: oai:DiVA.org:umu-146171DiVA, id: diva2:1202984
Available from: 2018-05-02 Created: 2018-05-02 Last updated: 2018-06-09Bibliographically approved

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Wågberg, Thomas

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