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Quantification of bound bicarbonate in photosystem II
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Chemistry, Molecular Biomimetics, Ångström Laboratory, Uppsala University, Uppsala, Sweden.
2018 (English)In: Photosynthetica (Praha), ISSN 0300-3604, E-ISSN 1573-9058, Vol. 56, no 1, p. 210-216Article in journal (Refereed) Published
Abstract [en]

In this study, we presented a new approach for quantification of bicarbonate (HCO3-) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and O-18-labelling, excludes the possibility of "non-accounted" HCO3- by avoiding (1) the employment of formate for removal of HCO3- from PSII, and (2) the extremely low concentrations of HCO3-/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3-, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 +/- 0.1 HCO3- are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3- molecule as ligand to the non-heme iron of PSII, while unbound HCO3- optimizes the water-splitting reactions by acting as a mobile proton shuttle.

Place, publisher, year, edition, pages
2018. Vol. 56, no 1, p. 210-216
Keywords [en]
hydrogen carbonate, inorganic carbon, mass spectrometry, Mn-stabilizing protein, non-heme iron, ygen-evolving complex
National Category
Biophysics
Identifiers
URN: urn:nbn:se:umu:diva-147475DOI: 10.1007/s11099-017-0758-4ISI: 000430309200019OAI: oai:DiVA.org:umu-147475DiVA, id: diva2:1203788
Funder
Swedish Research Council, 2016-05183Available from: 2018-05-04 Created: 2018-05-04 Last updated: 2018-06-09Bibliographically approved

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Shevela, DmitryMessinger, Johannes

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