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Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines
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2013 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 444, no 1-2, p. 1-11Article in journal (Refereed) Published
Abstract [en]

Hepatitis C virus (HCV) remains a major medical problem. In-depth study of HCV pathogenesis and immune responses is hampered by the lack of suitable small animal models. The narrow host range of HCV remains incompletely understood. We demonstrate that the entire HCV life-cycle can be recapitulated in mouse cells. We show that antiviral signaling interferes with HCV RNA replication in mouse cells. We were able to infect mouse cells expressing human CD81 and occludin (OCLN)-the minimal set of entry factor factors required for HCV uptake into mouse cells. Infected mouse cells sustain HCV RNA replication in the presence of miR122 and release infectious particles when mouse apoE is supplied. Our data demonstrate that the barriers of HCV interspecies transmission can be overcome by engineering a suitable cellular environment and provide a blue-print towards constructing a small animal model for HCV infection.

Place, publisher, year, edition, pages
San Diego: Academic Press, 2013. Vol. 444, no 1-2, p. 1-11
Keywords [en]
HCV animal model, Hepatitis C virus, Innate immunity, Species tropism, Viral assembly, Viral entry
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:umu:diva-148181DOI: 10.1016/j.virol.2013.05.036ISI: 000323873600001PubMedID: 23777661OAI: oai:DiVA.org:umu-148181DiVA, id: diva2:1210842
Available from: 2018-05-29 Created: 2018-05-29 Last updated: 2018-09-05Bibliographically approved

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Gerold, Gisa

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