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Selective concentration for ciprofloxacin resistance in Escherichia coli grown in complex aquatic bacterial biofilms
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2018 (English)In: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 116, p. 255-268Article in journal (Refereed) Published
Abstract [en]

There is concern that antibiotics in the environment can select for and enrich bacteria carrying acquired antibiotic resistance genes, thus increasing the potential of those genes to emerge in a clinical context. A critical question for understanding and managing such risks is what levels of antibiotics are needed to select for resistance in complex bacterial communities. Here, we address this question by examining the phenotypic and genotypic profiles of aquatic communities exposed to ciprofloxacin, also evaluating the within-species selection of resistant E. coli in complex communities. The taxonomic composition was significantly altered at ciprofloxacin exposure concentrations down to 1 mu g/L. Shotgun metagenomic analysis indicated that mobile quinolone resistance determinants (qnrD, qnrS and qnrB) were enriched as a direct consequence of ciprofloxacin exposure from 1 mu g/L or higher. Only at 5-10 mu g/L resistant E. coli increased relative to their sensitive counterparts. These resistant E. coli predominantly harbored non-transferrable, chromosomal triple mutations (gyrA S83 L, D87N and parC S80I), which confer high-level resistance. In a controlled experimental setup such as this, we interpret effects on taxonomic composition and enrichment of mobile quinolone resistance genes as relevant indicators of risk. Hence, the lowest observed effect concentration for resistance selection in complex communities by ciprofloxacin was 1 mu g/L and the corresponding no observed effect concentration 0.1 mu g/L. These findings can be used to define and implement discharge or surface water limits to reduce risks for selection of antibiotic resistance in the environment.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 116, p. 255-268
Keywords [en]
Antibiotic resistance, NOEC, LOEC, Environmental emission limits
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-148711DOI: 10.1016/j.envint.2018.04.029ISI: 000432745000029PubMedID: 29704804Scopus ID: 2-s2.0-85046122280OAI: oai:DiVA.org:umu-148711DiVA, id: diva2:1223447
Funder
Swedish Research Council, 2015-02492Swedish Research Council Formas, 942-2015-750; 2016-786Available from: 2018-06-25 Created: 2018-06-25 Last updated: 2018-06-25Bibliographically approved

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Fick, Jerker

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