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Realization of an optical tweezers set-up and evaluation of its stiffness using the equipartition theorem
Umeå University, Faculty of Science and Technology, Department of Physics.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The aim of this work was to build and optical tweezers instrument, trap a 1μm particle and calibrate the trapping stiffness. We used an oil- immersion objective to focus a He-Ne laser beam and to visualize the particle we used in a CCD camera. To measure the trapping stiffness, we used a position sensor detector (PSD) and measured the displacement of particle in microfluidic flow chamber, and transformed the motion to a power spectrum. To calibrate the trap, we applied the equipartition method to analyze trapped particles. The result shows that our instrument could trap particles and that its stiffness in x and y direction are 2.5(3) pN/μm and 2.8(3) pN/μm, respectively.

Place, publisher, year, edition, pages
2018.
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-150046OAI: oai:DiVA.org:umu-150046DiVA, id: diva2:1230759
Subject / course
Fysik C - Examensarbete
Available from: 2018-07-04 Created: 2018-07-04 Last updated: 2018-07-04Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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