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Realization of an optical tweezers set-up and evaluation of its stiffness using the power spectrum method
Umeå University, Faculty of Science and Technology, Department of Physics.
2018 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The aim of the work was to build an optical tweezers and evaluate its trapping stiffness using power spectrum method. In our work, a single beam optical tweezers system was constructed by using an inverted optical microscope system and then focusing light from a He-Ne laser with an objective with a high numerical aperture. Our setup can trap and manipulate polystyrene particles in three dimensions. We used this system to trap the polystyrene particles with diameters of 1μm in water. To measure the movements of the trapped particles we used a two-dimensional position sensitive detector (PSD) which we calibrated using the power spectrum method. The stiffness of the trap are 1.2(1) pN/μm and 1.4(2) pN/μm in the X and Y direction, respectively.

Place, publisher, year, edition, pages
2018.
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-150049OAI: oai:DiVA.org:umu-150049DiVA, id: diva2:1230786
Subject / course
Fysik C - Examensarbete
Available from: 2018-07-04 Created: 2018-07-04 Last updated: 2018-07-04Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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