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Cell Membrane Derived Platform To Study Virus Binding Kinetics and Diffusion with Single Particle Sensitivity
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2018 (Engelska)Ingår i: Acs Infectious Diseases, ISSN 2373-8227, Vol. 4, nr 6, s. 944-953Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Discovery and development of new antiviral therapies essentially rely on two key factors: an in-depth understanding of the mechanisms involved in viral infection and the development of fast and versatile drug screening platforms. To meet those demands, we present a biosensing platform to probe virus-cell membrane interactions on a single particle level. Our method is based on the formation of supported lipid bilayers from cell membrane material. Using total internal reflection fluorescence microscopy, we report the contribution of viral and cellular components to the interaction kinetics of herpes simplex virus type 1 with the cell membrane. Deletion of glycoprotein C (gC), the main viral attachment glycoprotein, or deletion of heparan sulfate, an attachment factor on the cell membrane, leads to an overall decrease in association of virions to the membrane and faster dissociation from the membrane. In addition to this, we perform binding inhibition studies using the antiviral compound heparin to estimate its IC50 value. Finally, single particle tracking is used to characterize the diffusive behavior of the virus particles on the supported lipid bilayers. Altogether, our results promote this platform as a complement to existing bioanalytical assays, being at the interface between simplified artificial membrane models and live cell experiments.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2018. Vol. 4, nr 6, s. 944-953
Nyckelord [en]
virus-membrane interactions, supported lipid bilayers, herpes simplex virus, binding kinetics, single particle tracking, biomimetic sensing platform
Nationell ämneskategori
Biofysik
Identifikatorer
URN: urn:nbn:se:umu:diva-150879DOI: 10.1021/acsinfecdis.7b00270ISI: 000435158700011PubMedID: 29688001OAI: oai:DiVA.org:umu-150879DiVA, id: diva2:1244301
Tillgänglig från: 2018-08-31 Skapad: 2018-08-31 Senast uppdaterad: 2019-05-23Bibliografiskt granskad

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Bally, Marta

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Avdelningen för virologiWallenberg centrum för molekylär medicin vid Umeå universitet (WCMM)
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