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Comparison of catalytically non-productive adsorption of fungal proteins to lignins and pseudo-lignin using isobaric mass tagging
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2018 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 268, p. 393-401Article in journal (Refereed) Published
Abstract [en]

Catalytically non-productive adsorption of fungal enzymes to pseudo-lignin (PL) was compared to adsorption to lignin preparations derived from different sources (SL, spruce; BL, birch; OL, beech) using different methods [steam pretreatment/enzymatic saccharification (SL, BL) and organosolv processing (OL)]. The protein adsorption to the SL was more extensive than the adsorption to the hardwood lignins, which was relatively similar to the adsorption to the PL. The adsorption patterns of 13 individual proteins were studied using isobaric mass tagging with TMTsixplex reagent and LC-MS/MS analysis. The results suggest that, on an average, adsorption of proteins equipped with carbohydrate-binding modules, such as the cellulases CBHI, EGII, and EGIV, was less dependent on the quality of the lignin/PL than adsorption of other proteins, such as beta-Xyl, Xyn-1, and Xyn-2, which are involved in xylan degradation.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 268, p. 393-401
Keywords [en]
Enzyme adsorption, Lignin, Pseudo-lignin, Quantitative proteomics, Isobaric mass tagging
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-152373DOI: 10.1016/j.biortech.2018.07.149ISI: 000445043600049PubMedID: 30099290OAI: oai:DiVA.org:umu-152373DiVA, id: diva2:1253763
Projects
Bio4Energy
Funder
Swedish Energy AgencyBio4EnergyThe Kempe FoundationsAvailable from: 2018-10-05 Created: 2018-10-05 Last updated: 2019-08-30Bibliographically approved

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Wang, ZhaoJönsson, Leif J.

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