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LEAFY activity is post-transcriptionally regulated by BLADE ON PETIOLE2 and CULLIN3 in Arabidopsis
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2018 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 220, no 2, p. 579-592Article in journal (Refereed) Published
Abstract [en]

The Arabidopsis LEAFY (LFY) transcription factor is a key regulator of floral meristem emergence and identity. LFY interacts genetically and physically with UNUSUAL FLORAL ORGANS, a substrate adaptor of CULLIN1-RING ubiquitin ligase complexes (CRL1). The functionally redundant genes BLADE ON PETIOLE1 (BOP1) and -2 (BOP2) are potential candidates to regulate LFY activity and have recently been shown to be substrate adaptors of CULLIN3 (CUL3)-RING ubiquitin ligases (CRL3). We tested the hypothesis that LFY activity is controlled by BOPs and CUL3s in plants and that LFY is a substrate for ubiquitination by BOP-containing CRL3 complexes. When constitutively expressed, LFY activity is fully dependent on BOP2 as well as on CUL3A and B to regulate target genes such as APETALA1 and to induce ectopic flower formation. We also show that LFY and BOP2 proteins interact physically and that LFY-dependent ubiquitinated species are produced invitro in a reconstituted cell-free CRL3 system in the presence of LFY, BOP2 and CUL3. This new post-translational regulation of LFY activity by CRL3 complexes makes it a unique transcription factor subjected to a positive dual regulation by both CRL1 and CRL3 complexes and suggests a novel mechanism for promoting flower development.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2018. Vol. 220, no 2, p. 579-592
Keywords [en]
BLADE-ON-PETIOLE, cullin 3, flower development, inflorescence, LEAFY, post-transcriptional regulation, ubiquitination
National Category
Environmental Sciences
Identifiers
URN: urn:nbn:se:umu:diva-152392DOI: 10.1111/nph.15329ISI: 000445194100022PubMedID: 29995985OAI: oai:DiVA.org:umu-152392DiVA, id: diva2:1254127
Funder
The Kempe FoundationsAvailable from: 2018-10-08 Created: 2018-10-08 Last updated: 2018-10-08Bibliographically approved

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Bakó, László

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Chahtane, HichamBakó, LászlóBenlloch, Reyes
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Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
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CiteExportLink to record
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