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Liquid-phase measurements of photosynthetic oxygen evolution
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Chemistry - Ångström Laboratory, Uppsala University, UppsalaSweden.
2018 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1770, p. 197-211Article in journal (Refereed) Published
Abstract [en]

This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.

Place, publisher, year, edition, pages
2018. Vol. 1770, p. 197-211
Keywords [en]
Photosynthetic water oxidation, O2 evolution, Photosystem II, Clark-type electrode, Membrane inlet mass spectrometry
National Category
Biophysics
Identifiers
URN: urn:nbn:se:umu:diva-152593DOI: 10.1007/978-1-4939-7786-4_11PubMedID: 29978403OAI: oai:DiVA.org:umu-152593DiVA, id: diva2:1255912
Available from: 2018-10-15 Created: 2018-10-15 Last updated: 2018-11-09Bibliographically approved

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Shevela, DmitriySchröder, Wolfgang P.

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