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Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and osteogenic differentiation group)
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). (Chondrogenic and osteogenic differentiation group)ORCID iD: 0000-0002-9294-7431
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Nordlab Kokkola, Keski-Pohjanmaa Central Hospital Soite, Kokkola, Finland. (Chondrogenic and osteogenic differentiation group)
Research Unit of Medical Imaging, Physics and Technology, University of Oulu, Oulu, Finland; Infotech Doctoral Program, University of Oulu, Oulu, Finland.
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2019 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878, Vol. 375, no 3, p. 629-639Article in journal (Refereed) Published
Abstract [en]

A lot has been invested into understanding how to assemble cartilage tissue in vitro and various designs have been developed to manufacture cartilage tissue with native-like biological properties. So far, no satisfactory design has been presented. Bovine primary chondrocytes are used to self-assemble scaffold-free constructs to investigate whether mechanical loading by centrifugal force would be useful in manufacturing cartilage tissue in vitro. Six million chondrocytes were laid on top of defatted bone disks placed inside an agarose well in 50-ml culture tubes. The constructs were centrifuged once or three times per day for 15 min at a centrifugal force of 771×g for up to 4 weeks. Control samples were cultured under the same conditions without exposure to centrifugation. The samples were analysed by (immuno)histochemistry, Fourier transform infrared imaging, micro-computed tomography, biochemical and gene expression analyses. Biomechanical testing was also performed. The centrifuged tissues had a more even surface covering a larger area of the bone disk. Fourier transform infrared imaging analysis indicated a higher concentration of collagen in the top and bottom edges in some of the centrifuged samples. Glycosaminoglycan contents increased along the culture, while collagen content remained at a rather constant level. Aggrecan and procollagen α1(II) gene expression levels had no significant differences, while procollagen α2(I) levels were increased significantly. Biomechanical analyses did not reveal remarkable changes. The centrifugation regimes lead to more uniform tissue constructs, whereas improved biological properties of the native tissue could not be obtained by centrifugation.

Place, publisher, year, edition, pages
New York: Springer, 2019. Vol. 375, no 3, p. 629-639
Keywords [en]
Cartilage tissue engineering, Centrifugal force, Osteoarthritis, Primary chondrocyte, Tissue assembly
National Category
Cell and Molecular Biology Orthopaedics Biochemistry and Molecular Biology Cell Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry; biomechanics; cell research; Orthopaedics
Identifiers
URN: urn:nbn:se:umu:diva-152816DOI: 10.1007/s00441-018-2938-3ISI: 000460535300006PubMedID: 30349935OAI: oai:DiVA.org:umu-152816DiVA, id: diva2:1261742
Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2019-04-08Bibliographically approved

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Prittinen, JuhaYlärinne, JannePiltti, JuhaLammi, MikkoQu, Chengjuan

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Cell and Tissue Research
Cell and Molecular BiologyOrthopaedicsBiochemistry and Molecular BiologyCell BiologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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