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The role of conserved proteins DrpA and DrpB in nitrate respiration of Thermus thermophilus
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0003-2429-7542
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2018 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 20, no 10, p. 3851-3861Article in journal (Refereed) Published
Abstract [en]

In many Thermus thermophilus strains, nitrate respiration is encoded in mobile genetic regions, along with regulatory circuits that modulate its expression based on anoxia and nitrate presence. The oxygen‐responsive system has been identified as the product of the dnrST (dnr) operon located immediately upstream of the nar operon (narCGHJIKT), which encodes the nitrate reductase (NR) and nitrate/nitrite transporters. In contrast, the nature of the nitrate sensory system is not known. Here, we analyse the putative nitrate‐sensing role of the bicistronic drp operon (drpAB) present downstream of the nar operon in most denitrifying Thermus spp. Expression of drp was found to depend on the master regulator DnrT, whereas the absence of DrpA or DrpB increased the expression of both DnrS and DnrT and, concomitantly, of the NR. Absence of both proteins made expression from the dnr and nar operons independent of nitrate. Polyclonal antisera allowed us to identify DrpA as a periplasmic protein and DrpB as a membrane protein, with capacity to bind to the cytoplasmic membrane. Here, we propose a role for DrpA/DrpB as nitrate sensors during denitrification.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018. Vol. 20, no 10, p. 3851-3861
National Category
Microbiology
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URN: urn:nbn:se:umu:diva-153131DOI: 10.1111/1462-2920.14400ISI: 000447549700029PubMedID: 30187633Scopus ID: 2-s2.0-85054313930OAI: oai:DiVA.org:umu-153131DiVA, id: diva2:1262167
Available from: 2018-11-09 Created: 2018-11-09 Last updated: 2018-11-09Bibliographically approved

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Alvarez, LauraCava, Felipe

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