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The MED7 subunit paralogs of Mediator function redundantly in development of etiolated seedlings in Arabidopsis
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Department of Biotechnology, Indira Gandhi National Tribal University (IGNTU), Amarkantak-484887, Madhya Pradesh,India.
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0003-1181-0415
2018 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 96, no 3, p. 578-594Article in journal (Refereed) Published
Abstract [en]

MED7 is a subunit of the Mediator middle module and is encoded by two paralogs in Arabidopsis. We generated MED7 silenced lines using RNAi to study its impact on Arabidopsis growth and development. Compared with wild type, etiolated seedlings of the MED7 silenced lines exhibited reduced hypocotyl length caused by reduced cell elongation when grown in the dark. The hypocotyl length phenotype was rescued by exogenously supplied brassinosteroid. In addition, MED7 silenced seedlings exhibited defective hook opening in the dark as well as defective cotyledon expansion in the presence of the brassinosteroid inhibitor brassinazole. Whole transcriptome analysis on etiolated seedlings using RNA sequencing revealed several genes known to be regulated by auxin and brassinosteroids, and a broad range of cell wall-related genes that were differentially expressed in the MED7 silenced lines. This was especially evident for genes involved in cell wall extension and remodeling, such as EXPANSINs and XTHs. Conditional complementation with each MED7 paralog individually restored the hypocotyl phenotype as well as the gene expression defects. Additionally, conditional expression of MED7 had no effects that were independent of the Mediator complex on the observed phenotypes. We concluded that the MED7 paralogs function redundantly in regulating genes required for the normal development of etiolated Arabidopsis seedlings.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018. Vol. 96, no 3, p. 578-594
Keywords [en]
Arabidopsis thaliana, MED7, Mediator complex, paralogs, skotomorphogenesis, transcriptional regulation
National Category
Forest Science
Identifiers
URN: urn:nbn:se:umu:diva-153697DOI: 10.1111/tpj.14052ISI: 000447881200008PubMedID: 30058106OAI: oai:DiVA.org:umu-153697DiVA, id: diva2:1268265
Funder
Swedish Cancer SocietySwedish Research CouncilKnut and Alice Wallenberg FoundationThe Kempe FoundationsCarl Tryggers foundation Available from: 2018-12-05 Created: 2018-12-05 Last updated: 2018-12-05Bibliographically approved

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Blomberg, JeanetteBjörklund, Stefan

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