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RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B
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2018 (English)In: RNA Biology, ISSN 1547-6286, E-ISSN 1555-8584, Vol. 15, no 10, p. 1336-1347Article in journal (Refereed) Published
Abstract [en]

Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′ untranslated region of speB mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity in vivo, we mutated it and demonstrate that the presence of this G residue is essential for the processing of the speB mRNA 5′ UTR by RNase Y. Although RNase Y directly targets and processes speB, we show that RNase Y-mediated regulation of speB expression occurs primarily at the transcriptional level and independently of the processing in the speB mRNA 5′ UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the speB 5′ UTR and on the role of RNase Y in speB regulation.

Place, publisher, year, edition, pages
Taylor & Francis, 2018. Vol. 15, no 10, p. 1336-1347
Keywords [en]
Streptococcus pyogenes, speB, RNase Y, virulence, 5' untranslated region, transcriptional regulation
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-154094DOI: 10.1080/15476286.2018.1532253ISI: 000450608900008PubMedID: 30290721OAI: oai:DiVA.org:umu-154094DiVA, id: diva2:1269882
Funder
Göran Gustafsson Foundation for Research in Natural Sciences and MedicineThe Kempe FoundationsSwedish Research Council, K2013-57X-21436-04-3Available from: 2018-12-11 Created: 2018-12-11 Last updated: 2018-12-11Bibliographically approved

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Le Rhun, AnaïsCharpentier, Emmanuelle

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Le Rhun, AnaïsCharpentier, Emmanuelle
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Molecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)Department of Molecular Biology (Faculty of Medicine)
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RNA Biology
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