umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Scale-up of production of bacterial nanocellulose using submerged cultivation
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Bioengineering, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, , Shanghai, China.ORCID iD: 0000-0002-6007-1180
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2018 (English)In: Journal of chemical technology and biotechnology (1986), ISSN 0268-2575, E-ISSN 1097-4660, Vol. 93, no 12, p. 3418-3427Article in journal (Refereed) Published
Abstract [en]

BACKGROUND More extensive utilization of bacterial nanocellulose (BNC) is severely restricted by the low efficiency and small scale of the traditional static cultivation. Submerged fermentation in stirred-tank reactors (STRs) is potentially favourable for large-scale production of BNC, but scale-up of cultivation remains challenging. Even though the STR is most commonly used for submerged cultivation in the fermentation industry, there are few previous attempts to scale-up production of BNC to pilot scale using an STR. Furthermore, the question of how scale-up of submerged cultivation affects the properties of the BNC has received very little attention.

RESULTS Four strains were compared in 250-mL shake flasks. Strain DHU-ATCC-1 displayed the highest volumetric productivity, 0.56 g L-1 d(-1), and was then cultivated in a 400-mL STR, showing a similar productivity of 0.55 g L-1 d(-1). Scale-up using a 75-L STR pilot bioreactor resulted in enhancement of the BNC production rate from 0.056 g d(-1) in the shake flasks to 17.3 g d(-1) in the 75-L STR, although the productivity decreased to 0.43 g L-1 d(-1). During scale-up from shake flasks to 400-mL STR and further on to 75-L STR, the BNC fibers formed more bundles, whereas the fiber diameter decreased from 25.6 to 21.7 nm. The BNC from the 75-L STR exhibited a higher degree of polymerization, specifically 3230, higher degree of crystallinity, specifically 83%, larger crystallites, and improved strength including higher tensile energy absorption index and superior stretch at break.

CONCLUSION It is possible to enhance BNC production, and maintain or improve its properties when scaling up submerged cultivation in STRs.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018. Vol. 93, no 12, p. 3418-3427
Keywords [en]
bacterial nanocellulose, Komagataeibacter xylinus, stirred-tank reactor, productivity, structure, mechanical property
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-154905DOI: 10.1002/jctb.5699ISI: 000451779600007OAI: oai:DiVA.org:umu-154905DiVA, id: diva2:1275047
Projects
Bio4EnergyAvailable from: 2019-01-04 Created: 2019-01-04 Last updated: 2019-08-30Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Chen, GenqiangWu, GuochaoJönsson, Leif J.

Search in DiVA

By author/editor
Chen, GenqiangWu, GuochaoJönsson, Leif J.
By organisation
Department of Chemistry
In the same journal
Journal of chemical technology and biotechnology (1986)
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 71 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf