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ATP-dependent force generation and membrane scission by ESCRT-III and Vps4
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2018 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 362, no 6421, p. 1423-+Article in journal (Refereed) Published
Abstract [en]

The endosomal sorting complexes required for transport (ESCRTs) catalyze reverse topology scission from the inner face of membrane necks in HIV budding, multivesicular endosome biogenesis, cytokinesis, and other pathways. We encapsulated ESCRT-III subunits Snf7, Vps24. and Vps2 and the AAA+ ATPase (adenosine triphosphatase) Vps4 in giant vesicles from which membrane nanotubes reflecting the correct topology of scission could be pulled. Upon ATP release by photo-uncaging, this system generated forces within the nanotubes that led to membrane scission in a manner dependent upon Vps4 catalytic activity and Vps4 coupling to the ESCRT-III proteins. Imaging of scission revealed Snf7 and Vps4 puncta within nanotubes whose presence followed ATP release, correlated with force generation and nanotube constriction, and preceded scission. These observations directly verify long-standing predictions that ATP-hydrolyzing assemblies of ESCRT-Ill and Vps4 sever membranes.

Place, publisher, year, edition, pages
American Association for the Advancement of Science , 2018. Vol. 362, no 6421, p. 1423-+
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-155095DOI: 10.1126/science.aat1839ISI: 000453845000063PubMedID: 30573630OAI: oai:DiVA.org:umu-155095DiVA, id: diva2:1277409
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NIH (National Institute of Health), R56AI127809NIH (National Institute of Health), R01GM032543NIH (National Institute of Health), R01AI112442Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved

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Carlson, Lars-Anders

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Carlson, Lars-Anders
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Department of Medical Biochemistry and BiophysicsWallenberg Centre for Molecular Medicine at Umeå University (WCMM)
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