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Flagella-mediated secretion of a novel Vibrio cholerae cytotoxin affecting both vertebrate and invertebrate hosts
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).ORCID iD: 0000-0002-7151-1137
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
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2018 (English)In: Communications Biology, ISSN 2399-3642, Vol. 1, article id 59Article in journal (Refereed) Published
Abstract [en]

Using Caenorhabditis elegans as an infection host model for Vibrio cholerae predator interactions, we discovered a bacterial cytotoxin, MakA, whose function as a virulence factor relies on secretion via the flagellum channel in a proton motive force-dependent manner. The MakA protein is expressed from the polycistronic makDCBA (motility-associated killing factor) operon. Bacteria expressing makDCBA induced dramatic changes in intestinal morphology leading to a defecation defect, starvation and death in C. elegans. The Mak proteins also promoted V. cholerae colonization of the zebrafish gut causing lethal infection. A structural model of purified MakA at 1.9 Å resolution indicated similarities to members of a superfamily of bacterial toxins with unknown biological roles. Our findings reveal an unrecognized role for V. cholerae flagella in cytotoxin export that may contribute both to environmental spread of the bacteria by promoting survival and proliferation in encounters with predators, and to pathophysiological effects during infections.

Place, publisher, year, edition, pages
Springer Nature Publishing AG , 2018. Vol. 1, article id 59
National Category
Microbiology in the medical area
Research subject
Infectious Diseases; Molecular Biology
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URN: urn:nbn:se:umu:diva-155563DOI: 10.1038/s42003-018-0065-zISI: 000461126500059PubMedID: 30271941OAI: oai:DiVA.org:umu-155563DiVA, id: diva2:1281515
Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2019-04-04Bibliographically approved

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Dongre, MiteshAung, Kyaw MinLarsson, PerMiftakhova, Regina R.Persson, Karinavon Hofsten, JonasPersson, Jenny L.Tuck, SimonUhlin, Bernt EricWai, Sun Nyunt

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Dongre, MiteshAung, Kyaw MinLarsson, PerMiftakhova, Regina R.Persson, Karinavon Hofsten, JonasPersson, Jenny L.Tuck, SimonUhlin, Bernt EricWai, Sun Nyunt
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Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)Department of ChemistryUmeå Centre for Molecular Medicine (UCMM)Department of Integrative Medical Biology (IMB)
Microbiology in the medical area

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