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A standardized protocol for comparable analysis of GSH/GSSG by UHPLC-ESI-MSMS for human plasma
Umeå University, Faculty of Social Sciences, Department of Psychology.ORCID iD: 0000-0001-5026-4934
Swedish Metabolomics Centre (SMC), Umeå, Sweden.
Swedish Metabolomics Centre (SMC), Umeå, Sweden.
Swedish Metabolomics Centre (SMC), Umeå, Sweden.
2019 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 1104, p. 67-72Article in journal (Refereed) Published
Abstract [en]

Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 different pre-processing procedures. For three of the samples an extraction mixture was added after 0 (baseline), 4 and 8 min and for three of the samples the extraction mixture was added at different times during defrost. A worst case scenario where a sample was left in a cool box during 6 h was also included. The samples were analyzed with UHPLC-ESIMSMS. A large difference in the levels of GSH and GSSG were identified and it was clearly associated with the sample handling procedures. A sample left untreated for 4 min will have significantly reduced amount of GSH. Stability tests showed that the level of GSH was reduced after 3 months in -80 degrees C.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 1104, p. 67-72
Keywords [en]
GIutathione, GSH, GSSG, Plasma, Sample pre-processing, UHPLC-ESI-MS
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-156612DOI: 10.1016/j.jchromb.2018.11.007ISI: 000456890000008PubMedID: 30445289OAI: oai:DiVA.org:umu-156612DiVA, id: diva2:1290348
Funder
Swedish Research Council Formas, 2014-1229Swedish Research Council Formas, 2016-1110Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-20Bibliographically approved

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Claeson, Anna-SaraGouveia-Figueira, Sandra C.

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