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Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus
Unité PMM, INSERM U786, Institut Pasteur, Paris, France, Universite de la Mediterranee, France.ORCID iD: 0000-0002-9915-002x
2011 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, no 9, article id e22876Article in journal (Refereed) Published
Abstract [en]

Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. These findings suggest that the dye enters viable cells through the pores. To our knowledge, this is the first demonstration that trypan blue may enter viable cells. Consequently, the use of trypan blue staining as a marker of vital status should be interpreted with caution. The blue coloration does not necessarily indicate cell lysis, but may rather indicate pore formation in the cell membranes and more generally increased membrane permeability.

Place, publisher, year, edition, pages
2011. Vol. 6, no 9, article id e22876
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Microbiology Immunology
Identifiers
URN: urn:nbn:se:umu:diva-156945DOI: 10.1371/journal.pone.0022876PubMedID: 21909398OAI: oai:DiVA.org:umu-156945DiVA, id: diva2:1293122
Available from: 2019-03-03 Created: 2019-03-03 Last updated: 2019-03-08Bibliographically approved

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Puhar, Andrea

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