umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Shigella flexneri type three secretion system effector IpgD inhibits T cell migration by manipulating host phosphoinositide metabolism
Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, 25–28 Rue du Dr Roux, 75724 Paris Cedex 15, France, and INSERM U786, Institut Pasteur, 25–28 Rue du Dr Roux, 75724 Paris Cedex 15, France . (Andrea Puhar)ORCID iD: 0000-0002-9915-002x
Show others and affiliations
2011 (English)In: Cell Host and Microbe, ISSN 1931-3128, E-ISSN 1934-6069, Vol. 9, no 4, p. 263-272Article in journal (Refereed) Published
Abstract [en]

Shigella, the Gram-negative enteroinvasive bacterium that causes shigellosis, relies on its type III secretion system (TTSS) and injected effectors to modulate host cell functions. However, consequences of the interaction between Shigella and lymphocytes have not been investigated. We show that Shigella invades activated human CD4(+) T lymphocytes. Invasion requires a functional TTSS and results in inhibition of chemokine-induced T cell migration, an effect mediated by the TTSS effector IpgD, a phosphoinositide 4-phosphatase. Remarkably, IpgD injection into bystander T cells can occur in the absence of cell invasion. Upon IpgD-mediated hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP(2)), the pool of PIP(2) at the plasma membrane is reduced, leading to dephosphorylation of the ERM proteins and their inability to relocalize at one T cell pole upon chemokine stimulus, likely affecting the formation of the polarized edge required for cell migration. These results reveal a bacterial TTSS effector-mediated strategy to impair T cell function.

Place, publisher, year, edition, pages
2011. Vol. 9, no 4, p. 263-272
National Category
Microbiology Immunology
Identifiers
URN: urn:nbn:se:umu:diva-156946DOI: 10.1016/j.chom.2011.03.010PubMedID: 21501826OAI: oai:DiVA.org:umu-156946DiVA, id: diva2:1293123
Available from: 2019-03-03 Created: 2019-03-03 Last updated: 2019-03-08Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Puhar, Andrea

Search in DiVA

By author/editor
Puhar, Andrea
In the same journal
Cell Host and Microbe
MicrobiologyImmunology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 31 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf