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NADH oxidation drives respiratory Na+ transport in mitochondria from Yarrowia lipolytica
Biochemisches Institut, Universität Zürich, Zurich, Switzerland, Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris Cedex 15, France.ORCID iD: 0000-0002-9915-002x
2008 (English)In: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 190, no 4, p. 471-480Article in journal (Refereed) Published
Abstract [en]

It is generally assumed that respiratory complexes exclusively use protons to energize the inner mitochondrial membrane. Here we show that oxidation of NADH by submitochondrial particles (SMPs) from the yeast Yarrowia lipolytica is coupled to protonophore-resistant Na+ uptake, indicating that a redox-driven, primary Na+ pump is operative in the inner mitochondrial membrane. By purification and reconstitution into proteoliposomes, a respiratory NADH dehydrogenase was identified which coupled NADH-dependent reduction of ubiquinone (1.4 micromol min(-1) mg(-1)) to Na+ translocation (2.0 micromol min(-1) mg(-1)). NADH-driven Na+ transport was sensitive towards rotenone, a specific inhibitor of complex I. We conclude that mitochondria from Y. lipolytica contain a NADH-driven Na+ pump and propose that it represents the complex I of the respiratory chain. Our study indicates that energy conversion by mitochondria does not exclusively rely on the proton motive force but may benefit from the electrochemical Na+ gradient established by complex I.

Place, publisher, year, edition, pages
2008. Vol. 190, no 4, p. 471-480
National Category
Microbiology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-156950DOI: 10.1007/s00203-008-0395-1PubMedID: 18551278OAI: oai:DiVA.org:umu-156950DiVA, id: diva2:1293127
Available from: 2019-03-03 Created: 2019-03-03 Last updated: 2019-03-11Bibliographically approved

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Puhar, Andrea

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