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Semisynthesis of autophagy protein LC3 conjugates
Chemical Genomics Centre of the Max Planck Society, Dortmund, Germany; Max-Planck-Institute of Molecular Physiology, Dortmund, Germany.
2017 (English)In: Bioorganic & Medicinal Chemistry, ISSN 0968-0896, E-ISSN 1464-3391, Vol. 25, no 18, p. 4971-4976Article in journal (Refereed) Published
Abstract [en]

Autophagy is a conserved catabolic process involved in the elimination of proteins, organelles and pathogens. Autophagosome formation is the key process in autophagy. Lipidated Atg8/LC3 proteins that are conjugated to phosphatidylethanolamine (PE) play a key role in autophagosome biogenesis. To understand the function of Atg8/LC3-PE in autophagosome formation and host-pathogen interaction requires preparation and structural manipulation of lipidated Atg8/LC3 proteins. Herein, we report the semisynthesis of LC3 proteins and mutants with modifications of different PE fragments or lipids using native chemical ligation and aminolysis approaches.

Place, publisher, year, edition, pages
Elsevier, 2017. Vol. 25, no 18, p. 4971-4976
Keywords [en]
Lipidated proteins, Native chemical ligation, Aminolysis, LC3, RavZ, Atg4, Autophagy
National Category
Biochemistry and Molecular Biology
Research subject
Biorganic Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-157490DOI: 10.1016/j.bmc.2017.05.051ISI: 000410725700007PubMedID: 28583805Scopus ID: 2-s2.0-85020068861OAI: oai:DiVA.org:umu-157490DiVA, id: diva2:1298423
Funder
EU, European Research Council, 678899Available from: 2019-03-22 Created: 2019-03-22 Last updated: 2019-03-22Bibliographically approved

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Wu, Yao-Wen

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CiteExportLink to record
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