umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ribosome profiling analysis of eEF3-depleted Saccharomyces cerevisiae
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).ORCID iD: 0000-0002-2970-2001
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).ORCID iD: 0000-0003-4966-1291
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0002-4861-4584
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).ORCID iD: 0000-0001-7117-7426
Show others and affiliations
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 3037Article in journal (Refereed) Published
Abstract [en]

In addition to the standard set of translation factors common in eukaryotic organisms, protein synthesis in the yeast Saccharomyces cerevisiae requires an ABCF ATPase factor eEF3, eukaryotic Elongation Factor 3. eEF3 is an E-site binder that was originally identified as an essential factor involved in the elongation stage of protein synthesis. Recent biochemical experiments suggest an additional function of eEF3 in ribosome recycling. We have characterised the global effects of eEF3 depletion on translation using ribosome profiling. Depletion of eEF3 results in decreased ribosome density at the stop codon, indicating that ribosome recycling does not become rate limiting when eEF3 levels are low. Consistent with a defect in translation elongation, eEF3 depletion causes a moderate redistribution of ribosomes towards the 5' part of the open reading frames. We observed no E-site codon-or amino acid-specific ribosome stalling upon eEF3 depletion, supporting its role as a general elongation factor. Surprisingly, depletion of eEF3 leads to a relative decrease in P-site proline stalling, which we hypothesise is a secondary effect of generally decreased translation and/or decreased competition for the E-site with eIF5A.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 9, article id 3037
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-157582DOI: 10.1038/s41598-019-39403-yISI: 000459891700047PubMedID: 30816176OAI: oai:DiVA.org:umu-157582DiVA, id: diva2:1300759
Available from: 2019-03-29 Created: 2019-03-29 Last updated: 2019-03-29Bibliographically approved

Open Access in DiVA

fulltext(5163 kB)45 downloads
File information
File name FULLTEXT01.pdfFile size 5163 kBChecksum SHA-512
bfd6ccd1d014c1b2cf49c490e399354968ea542e759e4078a39fb2bd853a8a318d2e53e75cb4465f56b26c425207b2ea1cdb530a8792d271f779c70108a84343
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Kasari, VilluMargus, TonuAtkinson, Gemma C.Johansson, Marcus J. O.Hauryliuk, Vasili

Search in DiVA

By author/editor
Kasari, VilluMargus, TonuAtkinson, Gemma C.Johansson, Marcus J. O.Hauryliuk, Vasili
By organisation
Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)Department of Molecular Biology (Faculty of Science and Technology)
In the same journal
Scientific Reports
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 45 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 232 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf