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Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and myosin heavy chain isoform expression
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB). Prosthodontics, Faculty of Dentistry, University of Toronto, Ontario, Canada.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB).
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2019 (English)In: Archives of Oral Biology, ISSN 0003-9969, E-ISSN 1879-1506, Vol. 99, p. 141-149Article in journal (Refereed) Published
Abstract [en]

Objective: to determine if tooth loss and dental implant placement in rats induce changes in the morphological and histochemical features of the Anterior Digastric muscle.

Design: Adult male Sprague-Dawley rats had their right maxillary molar teeth extracted. ‘Extraction-1’ and ‘Extraction-2 groups were sacrificed, respectively, 4 or 8 weeks later, and an Implant group had an implant placement 2 weeks after the molar extraction, and rats were sacrificed 3 weeks later (n = 4/group). Naive rats (n = 3) had no treatment. Morphometric and immunohistochemical techniques quantified Anterior Digastric muscle myofibres’ cross-sectional area (CSA) and myosin heavy chain (MyHC) isoform proportions. Significant ANOVAs were followed by post-hoc tests; p < 0.05 and 0.1 were considered to reflect levels of statistical significance.

Results: In naïve rats, the peripheral regions of the Anterior Digastric muscle was dominated by MyHC-IIx/b isoform and there were no MyHC-I isoforms; the central regions dominated by MyHC-IIx/b and MyHC-IIa isoforms. Compared with naive rats, tooth extraction produced, 8 (but not 4) weeks later, a decreased proportion of fast-contracting fatigue-resistant MyHC-IIa isoform (p = 0.08), and increased proportion of fast and intermediate fatigue-resistance MyHC-IIa/x/b isoform (p = 0.03). Dental implant placement following tooth extraction attenuated the extraction effects but produced a decreased proportion of fast-contracting fatiguable MyHC-llx/b isoform (p = 0.03) in the peripheral region, and increased inter-animal variability in myofibre-CSAs.

Conclusions: Given the crucial role that the Anterior Digastric muscle plays in many vital oral functions (e.g., chewing, swallowing), these changes may contribute to the changes in oral sensorimotor functions that occur in humans following such treatments.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 99, p. 141-149
Keywords [en]
Anterior digastric, Jaw-opening muscle, Masticatory muscle, Myosin heavy chain, Myofibre-type, Heterogeneity
National Category
Dentistry
Identifiers
URN: urn:nbn:se:umu:diva-157771DOI: 10.1016/j.archoralbio.2019.01.009ISI: 000460850900018PubMedID: 30684691Scopus ID: 2-s2.0-85060298074OAI: oai:DiVA.org:umu-157771DiVA, id: diva2:1302062
Available from: 2019-04-03 Created: 2019-04-03 Last updated: 2019-04-03Bibliographically approved

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Shah, FarhanStål, Per

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