umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate
Show others and affiliations
2019 (English)In: Journal of Proteomics, ISSN 1874-3919, E-ISSN 1876-7737, Vol. 197, p. 106-114Article in journal (Refereed) Published
Abstract [en]

Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. haemolyticus virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S. haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome.

The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins.

Data are available via ProteomeXchange with identifier PXD010389.

Biological significance: Clinical isolates of Staphylococcus haemolyticus are usually multidrug resistant, their main virulence factor is formation of biofilms, both factors leading to infections that are difficult to treat. We show that both clinical and commensal S. haemolyticusisolates produce membrane vesicles. Identification of staphylococcal membrane vesicles can potentially be used in novel approaches to combat staphylococcal infections, such as development of vaccines.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 197, p. 106-114
Keywords [en]
Staphylococcus haemolyticus, Opportunistic pathogen, Membrane, Vesicle cargo, Total secretome, Virulence factors
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-158067DOI: 10.1016/j.jprot.2018.11.013ISI: 000462108200011PubMedID: 30472255OAI: oai:DiVA.org:umu-158067DiVA, id: diva2:1304980
Available from: 2019-04-15 Created: 2019-04-15 Last updated: 2019-04-15Bibliographically approved

Open Access in DiVA

fulltext(2879 kB)39 downloads
File information
File name FULLTEXT01.pdfFile size 2879 kBChecksum SHA-512
da5af703de6e521d9bf044b0d6f946337d67d963af7e86ef6568321a46ff7c2b62f131aebd113b8b692526314f7461b85358e6bbc67e28850de82d3aa56c10bd
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Wai, Sun Nyunt

Search in DiVA

By author/editor
Bruun, Jack-AnsgarWai, Sun Nyunt
By organisation
Department of Molecular Biology (Faculty of Medicine)
In the same journal
Journal of Proteomics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 39 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 108 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf