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EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes
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2019 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 30, no 10, p. 1147-1159Article in journal (Refereed) Published
Abstract [en]

Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPAR.. Overall, these data suggest that EHD2 plays a key role for adipocyte function.

Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY , 2019. Vol. 30, no 10, p. 1147-1159
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Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-158942DOI: 10.1091/mbc.E18-10-0680ISI: 000466151300003PubMedID: 30811273OAI: oai:DiVA.org:umu-158942DiVA, id: diva2:1318386
Available from: 2019-05-27 Created: 2019-05-27 Last updated: 2019-05-27Bibliographically approved

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Lundmark, Richard

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CiteExportLink to record
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  • apa
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