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Comprehensive antimicrobial susceptibility testing of a large collection of clinical strains of Aggregatibacter actinomycetemcomitans does not identify resistance to amoxicillin
Aarhus University, Denmark.
Aarhus University, Denmark.
Umeå University, Faculty of Medicine, Department of Odontology. (Aa-gruppen)
Umeå University, Faculty of Medicine, Department of Odontology. (Aa-gruppen)ORCID iD: 0000-0002-8069-8263
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2019 (English)In: Journal of Clinical Periodontology, ISSN 0303-6979, E-ISSN 1600-051X, Vol. 46, no 8, p. 846-854Article in journal (Refereed) Published
Abstract [en]

AIM: The present study aims to determine the susceptibility of Aggregatibacter actinomycetemcomitans to amoxicillin by investigating a large collection of oral strains of diverse geographical origin.

METHODS: Two hundred and fifty-seven A. actinomycetemcomitans strains were serotyped using a multiplex polymerase chain reaction, and minimal inhibitory concentration (MIC) values of amoxicillin were determined using the agar dilution method (range 0.25 to 8.0 mg/L). The plates were spot-wise inoculated with approximately 104 colony-forming units, incubated in 5% CO2 at 37 C°, and visually inspected after 24 and 48 hours. A MIC ≤ 2.00 mg/L was categorised as susceptible using EUCAST interpretative criteria for Haemophilus species.

RESULTS: Amoxicillin MIC-values varied from 0.25 mg/L to 2.00 mg/L, and all tested strains, including strains previously reported as resistant, were susceptible to amoxicillin. The MIC50 was 1.00 mg/L and the MIC90 was 2.00 mg/L.

CONCLUSION: Meticulous investigation of strains including isolates previously reported as resistant could not confirm the emergence of resistance to β-lactams in A. actinomycetemcomitans. Based on the present in vitro results, amoxicillin can be considered a key oral antimicrobial agent for treatment of A. actinomycetemcomitans. This article is protected by copyright. All rights reserved.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019. Vol. 46, no 8, p. 846-854
Keywords [en]
EUCAST, Antimicrobial resistance, agar dilution method, breakpoints, β-lactams
National Category
Dentistry
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-159488DOI: 10.1111/jcpe.13148ISI: 000476508700007PubMedID: 31124155Scopus ID: 2-s2.0-85068110794OAI: oai:DiVA.org:umu-159488DiVA, id: diva2:1318891
Available from: 2019-05-28 Created: 2019-05-28 Last updated: 2019-08-12Bibliographically approved

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Claesson, RolfJohansson, Anders

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