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Structural isomers of the S-2 state in photosystem II: do they exist at room temperature and are they important for function?
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2019 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 166, no 1, p. 60-72Article in journal (Refereed) Published
Abstract [en]

In nature, an oxo‐bridged Mn4CaO5 cluster embedded in photosystem II (PSII), a membrane‐bound multi‐subunit pigment protein complex, catalyzes the water oxidation reaction that is driven by light‐induced charge separations in the reaction center of PSII. The Mn4CaO5 cluster accumulates four oxidizing equivalents to enable the four‐electron four‐proton catalysis of two water molecules to one dioxygen molecule and cycles through five intermediate S‐states, S0 – S4 in the Kok cycle. One important question related to the catalytic mechanism of the oxygen‐evolving complex (OEC) that remains is, whether structural isomers are present in some of the intermediate S‐states and if such equilibria are essential for the mechanism of the O‐O bond formation. Here we compare results from electron paramagnetic resonance (EPR) and X‐ray absorption spectroscopy (XAS) obtained at cryogenic temperatures for the S2state of PSII with structural data collected of the S1, S2 and S3 states by serial crystallography at neutral pH (∼6.5) using an X‐ray free electron laser at room temperature. While the cryogenic data show the presence of at least two structural forms of the S2 state, the room temperature crystallography data can be well‐described by just one S2 structure. We discuss the deviating results and outline experimental strategies for clarifying this mechanistically important question.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019. Vol. 166, no 1, p. 60-72
National Category
Physical Chemistry Biological Sciences
Identifiers
URN: urn:nbn:se:umu:diva-159873DOI: 10.1111/ppl.12947ISI: 000466108300007PubMedID: 30793319OAI: oai:DiVA.org:umu-159873DiVA, id: diva2:1322052
Conference
1st European Congress on Photosynthesis Research (EPS), JUN 25-28, 2018, Uppsala, SWEDEN
Funder
NIH (National Institute of Health), GM110501NIH (National Institute of Health), GM126289NIH (National Institute of Health), GM055302Swedish Research Council, 2016-05183
Note

Special Issue: SI

Available from: 2019-06-10 Created: 2019-06-10 Last updated: 2019-06-10Bibliographically approved

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de Lichtenberg, CasperMessinger, Johannes

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Gul, Sherazde Lichtenberg, CasperMessinger, JohannesKern, Jan
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Physiologia Plantarum: An International Journal for Plant Biology
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