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Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology. Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
Umeå University, Faculty of Medicine, Department of Clinical Microbiology. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
2019 (English)In: BIO-PROTOCOL, ISSN 2331-8325, Vol. 9, no 15, article id UNSP e3326Article in journal (Refereed) Published
Abstract [en]

Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans in a murine infection model. Hypoxia was measured using a fluorescent dye for carbonic anhydrase 9, a host enzyme active under hypoxic conditions. Emitted fluorescence was subsequently quantified using an IVIS system. This protocol was optimized for the use in subdermal infection in mice but has the potential to be adapted to other models of fungal infection.

Place, publisher, year, edition, pages
BIO-PROTOCOL , 2019. Vol. 9, no 15, article id UNSP e3326
Keywords [en]
Candida albicans, Subdermal infection, Hypoxia, IVIS, Mycology
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:umu:diva-162304DOI: 10.21769/BioProtoc.3326ISI: 000478805900012OAI: oai:DiVA.org:umu-162304DiVA, id: diva2:1349152
Available from: 2019-09-06 Created: 2019-09-06 Last updated: 2019-09-06Bibliographically approved

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Lopes, Jose PedroUrban, Constantin F.

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Clinical BacteriologyMolecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)Department of Clinical Microbiology
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