umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Genetic Profiling of Aggregatibacter actinomycetemcomitans Serotype B Isolated from Periodontitis Patients Living in Sweden
Umeå University, Faculty of Medicine, Department of Odontology. (Aa-gruppen)ORCID iD: 0000-0002-8069-8263
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology. (Aa-gruppen)
Show others and affiliations
2019 (English)In: Pathogens, ISSN 2076-0817, Vol. 8, no 3, p. 1-13, article id 153Article in journal (Refereed) Published
Abstract [en]

The bacterium Aggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis and with systemic diseases, such as endocarditis. By assessing a Ghanaian longitudinal adolescent cohort, we earlier recognized the cagE gene as a possible diagnostic marker for a subgroup of JP2 and non-JP2 genotype serotype b A. actinomycetemcomitans strains, associated with high leukotoxicity as determined in a semi-quantitative cell assay. This group of A. actinomycetemcomitans is associated with the progression of attachment loss. In the present work, we used conventional polymerase chain reaction (PCR) and quantitative PCR to perform the cagE genotyping of our collection of 116 selected serotype b A. actinomycetemcomitans strains, collected over a period of 15 years from periodontitis patients living in Sweden. The A. actinomycetemcomitans strains carrying cagE (referred to as cagE+; n = 49) were compared to the cagE-negative strains (n = 67), present at larger proportions in the subgingival plaque samples, and were also much more prevalent in the young (≤35 years) compared to in the old (>35 years) group of patients. Our present results underline the potential use of cagE genotyping in the risk assessment of the development of periodontal attachment loss in Swedish adolescents.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI, 2019. Vol. 8, no 3, p. 1-13, article id 153
Keywords [en]
Aggregatibacter actinomycetemcomitans; cagE; virB1; virB4; genotype; virulence
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Infectious Diseases
Identifiers
URN: urn:nbn:se:umu:diva-163485DOI: 10.3390/pathogens8030153ISI: 000488003600042PubMedID: 31533208OAI: oai:DiVA.org:umu-163485DiVA, id: diva2:1353389
Available from: 2019-09-23 Created: 2019-09-23 Last updated: 2019-10-24Bibliographically approved

Open Access in DiVA

fulltext(2228 kB)41 downloads
File information
File name FULLTEXT01.pdfFile size 2228 kBChecksum SHA-512
ac09b2c0759abd94ed8f230ac617bff2443d680efb07da8e2d2b44f764bb92a8c26f6d5905e44982c1a2a8beb9cf36783848c8dd25ef2d4d4ecafd71690fa0dc
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Johansson, AndersClaesson, RolfHöglund-Åberg, CarolaLindholm, MarkJasim, SarahOscarsson, Jan

Search in DiVA

By author/editor
Johansson, AndersClaesson, RolfHöglund-Åberg, CarolaLindholm, MarkJasim, SarahOscarsson, Jan
By organisation
Department of Odontology
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar
Total: 41 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 84 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf