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Digital holographic imaging with plane wavefronts – assessing the diffusing constant of a microparticle
Umeå University, Faculty of Science and Technology, Department of Physics.
2019 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Digital holographic microscopy is a useful tool to realize 3D particles tracking. In this work, we build an in-line holographic microscopy based on plane wave and record videos of the freely diffusing polystyrene particles. We used a tracking algorithm to determine the position and trajectory of particles with the diameter of 2±0.2μm each frame. From this trajectory, we obtained the diffusion coefficient of the particles in the x, y and z-direction, which were assessed to 1.868±0.038e-13 m2/s, 2.552±0.026e-13 m2/s and 0.851±1.045e-10 m2/s, respectively. Moreover, we wrote a MATLAB program to simulate Brownian motion of particles and discussed it with experimental results. The theorical and experimental values are basically same in the x and y-direction, but there is a large deviation in the z-direction.

Place, publisher, year, edition, pages
2019.
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:umu:diva-165559OAI: oai:DiVA.org:umu-165559DiVA, id: diva2:1373206
Subject / course
Fysik C - Examensarbete
Available from: 2019-11-26 Created: 2019-11-26 Last updated: 2019-11-26Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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