umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Multiple roles for Plasmodium berghei phosphoinositide-specific phospholipase C in regulating gametocyte activation and differentiation
2011 (English)In: Cellular Microbiology, ISSN 1462-5814, E-ISSN 1462-5822, Vol. 13, no 7, p. 955-966Article in journal (Refereed) Published
Abstract [en]

Critical events in the life cycle of malaria parasites are controlled by calcium-dependent signalling cascades, yet the molecular mechanisms of calcium release remain poorly understood. The synchronized development of Plasmodium berghei gametocytes relies on rapid calcium release from internal stores within 10 s of gametocytes being exposed to mosquito-derived xanthurenic acid (XA). Here we addressed the function of phosphoinositide-specific phospholipase C (PI-PLC) for regulating gametocyte activation. XA triggered the hydrolysis of PIP(2) and the production of the secondary messenger IP(3) in gametocytes. Both processes were selectively blocked by a PI-PLC inhibitor, which also reduced the early Ca(2+) signal. However, microgametocyte differentiation into microgametes was blocked even when the inhibitor was added up to 5 min after activation, suggesting a requirement for PI-PLC beyond the early mobilization of calcium. In contrast, inhibitors of calcium release through ryanodine receptor channels were active only during the first minute of gametocyte activation. Biochemical determination of PI-PLC activity was confirmed using transgenic parasites expressing a fluorescent PIP(2) /IP(3) probe that translocates from the parasite plasmalemma to the cytosol upon cell activation. Our study revealed a complex interdependency of Ca(2+) and PI-PLC activity, with PI-PLC being essential throughout gamete formation, possibly explaining the irreversibility of this process.

Place, publisher, year, edition, pages
Blackwell Publishing Ltd , 2011. Vol. 13, no 7, p. 955-966
Keywords [en]
Animals, Calcium/metabolism, Female, *Host-Pathogen Interactions, Inositol 1, 4, 5-Trisphosphate/metabolism, Mice, Models, Biological, Phosphatidylinositol 4, 5-Diphosphate/metabolism, Phosphoinositide Phospholipase C/*metabolism, Plasmodium berghei/*enzymology/growth & development/*pathogenicity, Xanthurenates/metabolism
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-165872DOI: 10.1111/j.1462-5822.2011.01591.xOAI: oai:DiVA.org:umu-165872DiVA, id: diva2:1379356
Note

21518218[pmid]; PMC3132445[pmcid]

Available from: 2019-12-17 Created: 2019-12-17 Last updated: 2019-12-17

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full texthttps://www.ncbi.nlm.nih.gov/pubmed/21518218
In the same journal
Cellular Microbiology
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 4 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf