umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Genetic and transcriptional analysis of phosphoinositide-specific phospholipase C in Plasmodium
Show others and affiliations
2011 (English)In: Experimental parasitology, ISSN 0014-4894, E-ISSN 1090-2449, Vol. 129, no 1, p. 75-80Article in journal (Refereed) Published
Abstract [en]

Phosphoinositide-specific phospholipase C (PI-PLC) is a major regulator of calcium-dependent signal transduction, which has been shown to be important in various processes of the malaria parasite Plasmodium. PI-PLC is generally implicated in calcium liberation from intracellular stores through the action of its product, inositol-(1,4,5)-trisphosphate, and is itself dependent on calcium for its activation. Here we describe the plc genes from Plasmodium species. The encoded proteins contain all domains typically found in PI-PLCs of the δ class but are almost twice as long as their orthologues in mammals. Transcriptional analysis by qRT-PCR of plc during the erythrocytic cycle of P. falciparum revealed steady expression levels that increased at the late schizont stages. Genetic analysis in the P. berghei model revealed that the plc locus was targetable but that plc gene knock-outs could not be obtained, thereby strongly indicating that the gene is essential during blood stage development. Alternatively, we attempted to modify plc expression through a promoter exchange approach but found the gene to be refractory to over-expression indicating that plc expression levels might additionally be tightly controlled.

Place, publisher, year, edition, pages
2011. Vol. 129, no 1, p. 75-80
Keywords [en]
Amino Acid Sequence, Animals, Gene Expression Regulation, Enzymologic, Humans, Mice, Phosphoinositide Phospholipase C/chemistry/*genetics/metabolism, Plasmodium berghei/*enzymology/genetics, Plasmodium falciparum/*enzymology/genetics, Sequence Alignment, Transcriptional Activation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-165870DOI: 10.1016/j.exppara.2011.05.023PubMedID: 21651909OAI: oai:DiVA.org:umu-165870DiVA, id: diva2:1379360
Conference
United States
Available from: 2019-12-17 Created: 2019-12-17 Last updated: 2019-12-20Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Billker, Oliver

Search in DiVA

By author/editor
Billker, Oliver
In the same journal
Experimental parasitology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 9 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf